A color filter substrate includes a transparent substrate, a light-blocking layer, a color filter layer, a first cell gap maintaining member and a second cell gap maintaining member. The transparent substrate has a plurality of pixel regions. Each of the pixel regions includes first and second regions. The light-blocking layer is disposed over the transparent substrate. The light-blocking layer blocks light that leaks through boundaries of the pixel regions. The color filter layer is disposed over the transparent substrate. The color filter layer has a first thickness at the first region and a second thickness that is smaller than the first thickness at the second region. The first cell gap maintaining member is disposed at the first region. The second cell gap maintaining member is disposed at the second region. Therefore, a height difference between the main column spacer and the sub column spacer may be easily adjusted.