Invention Grant
- Patent Title: Method for linear mRNA amplification
- Patent Title (中): 线性mRNA扩增方法
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Application No.: US11179813Application Date: 2005-07-11
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Publication No.: US07927838B2Publication Date: 2011-04-19
- Inventor: Karen W. Shannon
- Applicant: Karen W. Shannon
- Applicant Address: US CA Santa Clara
- Assignee: Agilent Technologies, Inc.
- Current Assignee: Agilent Technologies, Inc.
- Current Assignee Address: US CA Santa Clara
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C12P19/34 ; C07H21/04

Abstract:
Methods for linearly amplifying mRNA to produce antisense RNA are provided. In the subject methods, mRNA is converted to double-stranded cDNA using a promoter-primer having a poly-dT primer site linked to a promoter sequence so that the resulting double-stranded cDNA is recognized by an RNA polymerase. The resultant double-stranded cDNA is then transcribed into antisense RNA in the presence of a reverse transcriptase that is rendered incapable of RNA-dependent DNA polymerase activity during this transcription step. The subject methods find use a variety of different applications in which the preparation of linearly amplified amounts of antisense RNA is desired. Also provided are kits for practicing the subject methods.
Public/Granted literature
- US20060024799A1 Method for linear mRNA amplification Public/Granted day:2006-02-02
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