Invention Grant
- Patent Title: High fidelity DNA polymerase compositions and uses therefor
- Patent Title (中): 高保真DNA聚合酶组合物及其用途
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Application No.: US10227110Application Date: 2002-08-23
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Publication No.: US07932070B2Publication Date: 2011-04-26
- Inventor: Holly Hogrefe , Michael Borns , Joseph A. Sorge
- Applicant: Holly Hogrefe , Michael Borns , Joseph A. Sorge
- Applicant Address: US CA Santa Clara
- Assignee: Agilent Technologies, Inc.
- Current Assignee: Agilent Technologies, Inc.
- Current Assignee Address: US CA Santa Clara
- Main IPC: C12N9/12
- IPC: C12N9/12 ; C07K1/00
Abstract:
The subject invention relates to compositions comprising an enzyme mixture which comprises a first enzyme and a second enzyme, where the first enzyme comprises a DNA polymerization activity and the second enzyme comprises an 3′-5′ exonuclease activity and a reduced DNA polymerization activity. The invention also relates to the above compositions in kit format and methods for high fidelity DNA synthesis using the subject compositions of the invention.
Public/Granted literature
- US20030143577A1 High fidelity DNA polymerase compositions and uses therefor Public/Granted day:2003-07-31
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