Invention Grant
- Patent Title: Intracellular production of a nuclease
- Patent Title (中): 核酸酶的细胞内产生
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Application No.: US11660110Application Date: 2005-08-12
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Publication No.: US07935509B2Publication Date: 2011-05-03
- Inventor: Pei-Chung Hsieh
- Applicant: Pei-Chung Hsieh
- Applicant Address: US MA Ipswich
- Assignee: New England Biolabs, Inc.
- Current Assignee: New England Biolabs, Inc.
- Current Assignee Address: US MA Ipswich
- Agent Harriet M. Strimpel
- International Application: PCT/US2005/028739 WO 20050812
- International Announcement: WO2006/020868 WO 20060223
- Main IPC: C12N9/16
- IPC: C12N9/16 ; C12N9/22 ; C12Q1/34 ; C12Q1/68 ; C07K14/00 ; C12P21/00
Abstract:
Methods and compositions are provided that relate to obtaining a recombinant DNA and RNA cleaving nuclease. This involves the over-expression of a fusion protein between maltose-binding protein and a truncated nuclease in a soluble form in the cytoplasm of a host cell from which it can be readily extracted.
Public/Granted literature
- US20080118949A1 Intracellular Production Of A Nuclease Public/Granted day:2008-05-22
Information query
