Invention Grant
- Patent Title: Immunochromatography method using fragmented antibody
- Patent Title (中): 使用片段化抗体的免疫色谱法
-
Application No.: US12239640Application Date: 2008-09-26
-
Publication No.: US07935541B2Publication Date: 2011-05-03
- Inventor: Hiroyuki Chiku
- Applicant: Hiroyuki Chiku
- Applicant Address: JP Tokyo
- Assignee: Fujifilm Corporation
- Current Assignee: Fujifilm Corporation
- Current Assignee Address: JP Tokyo
- Agency: Birch, Stewart, Kolasch & Birch, LLP
- Priority: JP2007-254173 20070928; JP2008-029988 20080212
- Main IPC: G01N33/553
- IPC: G01N33/553

Abstract:
An object of the present invention is to overcome the problem of nonspecific adsorption of a labeled antibody on the detection site and to provide an immunochromatography method by which the false positive signal can be suppressed, and wherein the detection sensitivity is high and the highly reliable measurement can be performed. The present invention provides an immunochromatography method, which comprises developing an analyte and a labeling substance modified with a first antibody against the analyte on a porous carrier in a state where the analyte and the labeling substance is mixed, and capturing the analyte and the labeling substance at the reaction site on the porous carrier having a second antibody against the analyte, so as to detect the analyte; wherein a fragmented antibody is used as the first antibody and/or the second antibody; an analyte is detected via amplification using an amplification solution which contains a compound containing silver and a reducing agent for silver ions; and the ratio of the non-specifically adsorbed labeling substance in the pre-amplification detection site and the non-detection site of the porous carrier is between 0.4 to 2.5 in a system where the density of the non-specifically adsorbed labeling substance is 106/mm2 or less.
Public/Granted literature
- US20090087927A1 IMMUNOCHROMATOGRAPHY METHOD USING FRAGMENTED ANTIBODY Public/Granted day:2009-04-02
Information query
IPC分类: