The present application is directed to thermoelectric coolers that include multiple temperature zones. The thermoelectric cooler may include a first series of thermoelectric intermediate members interconnecting a second plate with a first section of a first plate, and a second series of thermoelectric intermediate members interconnecting the second plate with a second section of the first plate. The first series may form a first temperature zone and the second series may form a second temperature zone. Each of the first and second series of thermoelectric intermediate members may be configured to electrically connect with a DC power supply to energize the first and second series and independently control temperatures of the first and second sections of the second plate.