Invention Grant
US07947312B2 Method of preparation and composition of a water soluble extract of the bioactive component of the plant species Uncaria for enhancing immune, anti-inflammatory, anti-tumor and DNA repair processes of warm blooded animals
有权
用于增强温血动物的免疫,抗炎,抗肿瘤和DNA修复过程的植物物种Uncaria的生物活性成分的水溶性提取物的制备方法和组合物
- Patent Title: Method of preparation and composition of a water soluble extract of the bioactive component of the plant species Uncaria for enhancing immune, anti-inflammatory, anti-tumor and DNA repair processes of warm blooded animals
- Patent Title (中): 用于增强温血动物的免疫,抗炎,抗肿瘤和DNA修复过程的植物物种Uncaria的生物活性成分的水溶性提取物的制备方法和组合物
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Application No.: US12473396Application Date: 2009-05-28
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Publication No.: US07947312B2Publication Date: 2011-05-24
- Inventor: Ronald W. Pero
- Applicant: Ronald W. Pero
- Agency: Davidoff Malito & Hutcher LLP
- Agent David W. Danenberg
- Main IPC: A61K36/00
- IPC: A61K36/00 ; A61K31/34 ; A61K31/05

Abstract:
The disclosure provides a method for isolating the bioactive component of the water-soluble extract of Uncaria tomentosa known as C-MED-100®, comprising (i) precipitating the spray drying carrier from C-MED-100®; (ii) using the resulting C-MED-100® to obtain a spotting mixture for thin layer chromatography (TLC); (iii) spotting the C-MED-100® spotting mixture on pre-run TLC plates and eluting the plates to obtain the fluorescing band with Rf=0.2-0.3; (iv) scraping off the Rf=0.2-0.3 band, eluting it in ammonia and freeze drying the eluted band to form a powder; and (v) extracting the powder with methanol to remove solubilized silica gel, concentrating the methanol solution and crystallizing the concentrated solution to obtain the bioactive component. The isolated bioactive component in vitro is a quinic acid analog, preferably quinic acid lactone. By contrast, the disclosure further shows the isolated bioactive component in vivo is quinic acid, whether as free acid or as a quinic acid salt, including quinic acid ammonium salt. The disclosure then provides for a pharmaceutical composition comprising a pharmaceutically effective amount of the bioactive component and a nontoxic inert carrier or diluent. The pharmaceutical composition and bioactive component may be used to enhance immune competency, treat disorders associated with the immune system, inhibit the inflammatory response, treat disorders associated with the inflammatory response, enhance the anti-tumor response, and treat disorders associated with the response to tumor formation and growth, all in mammals.
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