Invention Grant
US07947501B2 Gene recombination exchange system for stable gene modification in human ES cells
有权
用于人ES细胞稳定基因修饰的基因重组交换系统
- Patent Title: Gene recombination exchange system for stable gene modification in human ES cells
- Patent Title (中): 用于人ES细胞稳定基因修饰的基因重组交换系统
-
Application No.: US12322809Application Date: 2009-02-06
-
Publication No.: US07947501B2Publication Date: 2011-05-24
- Inventor: Su-Chun Zhang , Zhong-wei Du
- Applicant: Su-Chun Zhang , Zhong-wei Du
- Applicant Address: US WI Madison
- Assignee: Wisconsin Alumni Research Foundation
- Current Assignee: Wisconsin Alumni Research Foundation
- Current Assignee Address: US WI Madison
- Agency: Quarles & Brady, LLP
- Main IPC: C12N15/87
- IPC: C12N15/87
Abstract:
A method of creating a human pluripotent transgenic stem cell, wherein heterologous DNA is inserted into specific “hot-spots” in the genome where stable and high gene expression may occur, is disclosed. In one embodiment, the method comprises the steps of: (a) selecting a pluripotent stem cell line, and (b) inserting heterologous DNA at an insertion site selected from the group consisting of insertion site one and insertion site two to form a transgenic cell line. In another embodiment, the heterologous DNA is an exchange cassette and the transgenic cell line formed is a master cell line.
Public/Granted literature
- US20090239305A1 Gene recombination exchange system for stable gene modification in human ES cells Public/Granted day:2009-09-24
Information query
IPC分类:
