Invention Grant
US07947821B2 Optimized DNA sequences encoding recombinant human bone morphogenetic protein-2 (rhBMP-2), preparation method and the uses thereof
有权
编码重组人骨形态发生蛋白-2（rhBMP-2）的优化DNA序列，其制备方法及其用途
- Patent Title: Optimized DNA sequences encoding recombinant human bone morphogenetic protein-2 (rhBMP-2), preparation method and the uses thereof
- Patent Title (中): 编码重组人骨形态发生蛋白-2（rhBMP-2）的优化DNA序列，其制备方法及其用途
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Application No.: US12623527Application Date: 2009-11-23
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Publication No.: US07947821B2Publication Date: 2011-05-24
- Inventor: Changshen Liu , Jun Lin , Jiangchao Qian , Yuan Yuan
- Applicant: Changshen Liu , Jun Lin , Jiangchao Qian , Yuan Yuan
- Applicant Address: CN Shanghai
- Assignee: Shanghai Rebone Biomaterials Co., Ltd.
- Current Assignee: Shanghai Rebone Biomaterials Co., Ltd.
- Current Assignee Address: CN Shanghai
- Agency: Hamre, Schumann, Mueller & Larson, P.C.
- Priority: CN200910045832 20090123
- Main IPC: C07H21/02
- IPC: C07H21/02 ; C07H21/04 ; C12N15/63 ; C12N15/85 ; C12N15/86 ; C12N1/20
Abstract:
The invention discloses an optimized DNA sequence of recombinant human bone morphogenetic protein-2 (rhBMP-2) based on the Escherichia coli expression system and a method for the preparation of the rhBMP-2. Specifically, the invention provides the optimal DNA sequences suitable for Escherichia coli expression system, the methods for efficient preparation of the rhBMP-2, and the related construction of the recombinant bacteria, the expression and purification technologies. Compared with the traditional hBMP-2 gene without optimizing, the rhBMP-2 expression level of the optimized gene in Escherichia coli is increased by 50%. Additionally, this invention also provides a method for preparation long chain rhBMP-2 with enhanced renaturation efficiency and yield of purification.
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