System and method for data synchronization in Passive Optical Networks. According to an embodiment, the present invention provides a method for providing upstream data synchronization in an optical communication network. The method includes sending data from an Optical Network Unit. The data includes a first data frame, which includes a header sequence, a synchronization segment, and a data segment. The synchronization segment includes 66 bits, which includes a first number of bits having nonzero values and a second number of bits having a value of zero. The first number is different from the second number. The method further includes receiving at least the first data frame by an Optical Line Terminal. The method also includes processing the first data frame. The method additionally includes selecting a first segment of the first data frame, the first segment including 66 bits.