Methods, apparatus and systems for measuring a thickness of a portion of a multi-layer vessel based on at least one medical diagnostic image frame using an integrated ultrasound device is provided. The method includes for each x-coordinate determining a y-coordinate along an interface between each layer of the vessel including determining a Y coordinate of a center of an intima-media center (IMC) determining the adventitia-media interface Y coordinate, determining the intima-lumen interface Y coordinate, determining for each X coordinate the lumen-intima interface Y coordinate, determining media-adventitia interface Y coordinate, determining a thickness of at least one vessel layer using X and Y coordinates of respective layer interfaces, and outputting the thickness to a display.