Invention Grant
US07955793B2 Cultured silkworm cells capable of highly efficient baculovirus production and protein production
有权
培养能够高效率的杆状病毒生产和蛋白质生产的蚕细胞
- Patent Title: Cultured silkworm cells capable of highly efficient baculovirus production and protein production
- Patent Title (中): 培养能够高效率的杆状病毒生产和蛋白质生产的蚕细胞
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Application No.: US12226689Application Date: 2007-04-26
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Publication No.: US07955793B2Publication Date: 2011-06-07
- Inventor: Takahiro Kusakabe , Chisa Aoki , Osamu Ninagi , Jae Man Lee , Kazuhiro Iiyama , Naoya Kawakami
- Applicant: Takahiro Kusakabe , Chisa Aoki , Osamu Ninagi , Jae Man Lee , Kazuhiro Iiyama , Naoya Kawakami
- Applicant Address: JP Fukuoka-shi
- Assignee: Kyushu University, National University Corporation
- Current Assignee: Kyushu University, National University Corporation
- Current Assignee Address: JP Fukuoka-shi
- Agency: Birch, Stewart, Kolasch & Birch, LLP
- Priority: JP2006-122943 20060427
- International Application: PCT/JP2007/059028 WO 20070426
- International Announcement: WO2007/125982 WO 20071108
- Main IPC: C12Q1/70
- IPC: C12Q1/70
Abstract:
Known cell lines derived from silkworm exhibit low propagation efficiency of BmNPV. Accordingly, systems using known culture cell lines derived from silkworms take a long time to establish recombinant viruses, and are not suitable for preparation of virus solutions with high titers. The present invention provides a cell line Bme21 (FERM P-20852) that is derived from a silkworm embryo and is highly susceptible to BmNPV or its variant having the same biological characteristics. The present invention also provides a method of producing a recombinant virus, a method of producing a recombinant protein, a method of increasing efficiency of recombinant virus production, and a method of increasing efficiency of recombinant protein production, using the cell line Bme21 or its variant.
Public/Granted literature
- US20090305385A1 Novel Cultured Silkworm Cells Capable of Highly Efficient Baculovirus Production and Protein Production Public/Granted day:2009-12-10
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