Invention Grant
US07955832B2 Composition useful for protein fragment complementation assays (PCA) using fragments of E. coli/TEM-1 β-lactamase
失效
用于使用大肠杆菌/ TEM-1和β-内酰胺酶片段的蛋白质片段互补测定（PCA）的组合物
- Patent Title: Composition useful for protein fragment complementation assays (PCA) using fragments of E. coli/TEM-1 β-lactamase
- Patent Title (中): 用于使用大肠杆菌/ TEM-1和β-内酰胺酶片段的蛋白质片段互补测定（PCA）的组合物
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Application No.: US11002259Application Date: 2004-12-03
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Publication No.: US07955832B2Publication Date: 2011-06-07
- Inventor: Stephen William Watson Michnick , André Galarneau
- Applicant: Stephen William Watson Michnick , André Galarneau
- Applicant Address: US CA San Ramon
- Assignee: ODYSSEY THERA Inc.
- Current Assignee: ODYSSEY THERA Inc.
- Current Assignee Address: US CA San Ramon
- Agent Isaac A. Angres
- Main IPC: C12N9/78
- IPC: C12N9/78
Abstract:
The present invention describes an assay method comprising: (A) generating (1) at least a first fragment of a reporter molecule linked to a first interacting domain and at least a second fragment of a reporter molecule linked to a second interacting domain, or (2) nucleic acid molecules that code for (A)(1) and subsequently allowing said nucleic acid molecules to produce their coded products; then, (B) allowing interaction of said domains; and (C) detecting reconstituted reporter molecule activity, where said reporter molecule can react with a penicillin- or a cephalosporin-class substrate.
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