In this invention is described a method that foresees the isolation of a new subpopulation of stem cells derived form dental pulp, whose differentiation is osteoblasts lead to the subsequent production and employment of a bone tissue, called LAB (Living Autologous Bone). Specifically, the invention describes: 1) the isolation of stem cells from the pulp of deciduous and permanent teeth and of dental germs, obtained from human subjects; 2) the growth of these cells in vitro, under specific conditions that allow the isolation of a cellular sub-population, which, after differentiation in osteoblasts, is able to produce in vitro an extracellular matrix, identical to that detectable in bone tissue; 3) the use of this selected and differentiated cell population in order to produce autologous bone tissue in vitro, containing vital osteoblasts; 4) the preservation of the LAB under conditions which guarantee cellular vitality; 5) the use of the LAB in donor patients to reconstruct bone tissue, as required in the daily practice in dentistry, maxillo-facial surgery and orthopedics.