According to the invention, there is provided a novel soybean variety designated RJS53001. This invention thus relates to the seeds of soybean variety RJS53001, to the plants of soybean RJS53001 to plant parts of soybean variety RJS53001 and to methods for producing a soybean plant produced by crossing plants of the soybean variety RJS53001 with another soybean plant, using RJS53001 as either the male or the female parent.