Invention Grant
US07972847B2 Mature type-1 polarized dendritic cells with enhanced IL-12 production and methods of serum-free production and use
失效
具有增强的IL-12产生的成熟的1型偏振树突状细胞和无血清生产和使用的方法
- Patent Title: Mature type-1 polarized dendritic cells with enhanced IL-12 production and methods of serum-free production and use
- Patent Title (中): 具有增强的IL-12产生的成熟的1型偏振树突状细胞和无血清生产和使用的方法
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Application No.: US12138324Application Date: 2008-06-12
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Publication No.: US07972847B2Publication Date: 2011-07-05
- Inventor: Pawel Kalinski
- Applicant: Pawel Kalinski
- Agency: Klarquist Sparkman, LLP
- Main IPC: C12N5/071
- IPC: C12N5/071 ; C12N5/00 ; C12N5/02
Abstract:
The present invention discloses novel dendritic cell maturation-inducing cytokine cocktails, and methods for inducting type-1 polarized dendritic cells in serum-free conditions which enhance the desirable properties of DC1s generated in serum-supplemented cultures. The invention further discloses methods and systems using IFNγ and other ligands of the IFNγreceptor, in combination with IFNα (or other type I interferons), poly I:C, and other IFNα (and IFNβ) inducers to enhance the IL-12-producing properties of dendritic cells. More specifically, the present invention discloses type-1 polarized dendritic cells that have a unique combination of a fully-mature status and an elevated, instead of “exhausted”, ability to produce IL-12p70 allows for the generation of fully-mature DC1s in serum-free AIM-V medium. The invention discloses systems that use the foregoing products and methods to facilitate the clinical application of DC1-based vaccines and the identification of novel factors involved in the induction of Th1 and CTL responses by DC1.
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