Invention Grant
US07972872B2 Chromatography detection apparatus, detection method, and kit utilizing the same 有权
色谱检测装置,检测方法和使用其的试剂盒

  • Patent Title: Chromatography detection apparatus, detection method, and kit utilizing the same
  • Patent Title (中): 色谱检测装置,检测方法和使用其的试剂盒
  • Application No.: US11628621
    Application Date: 2005-06-07
  • Publication No.: US07972872B2
    Publication Date: 2011-07-05
  • Inventor: Ryo ShidaHiromi Ito
  • Applicant: Ryo ShidaHiromi Ito
  • Applicant Address: JP Tokyo
  • Assignee: Denka Seiken Co., Ltd.
  • Current Assignee: Denka Seiken Co., Ltd.
  • Current Assignee Address: JP Tokyo
  • Agency: Foley & Lardner LLP
  • Agent Stephen A. Bent
  • Priority: JP2004-169200 20040607
  • International Application: PCT/JP2005/010386 WO 20050607
  • International Announcement: WO2005/121794 WO 20051222
  • Main IPC: G01N33/53
  • IPC: G01N33/53
Chromatography detection apparatus, detection method, and kit utilizing the same
Abstract:
This invention provides an immunochromatography detection apparatus exhibiting excellent convenience, sensitivity, and specificity, a detection method therefore, a kit using the same, and a method for producing such apparatus and kit, by constructing a solid-phase support, on which the conditions for the reaction wherein an analyte specifically binds to a labeled reagent containing a ligand that specifically binds to the analyte and those for the reaction wherein a capture reagent specifically binds to a complex of the analyte and the labeled reagent in the immunochromatography detection method are continuously optimized, and the pretreated specimens are optimized for such reactions. This chromatography detection apparatus comprises: a sheet-like solid-phase support; a specimen-supply site thereon at which a specimen deduced to contain at least an analyte or a mixture of such specimen and a labeled reagent containing a ligand that specifically binds to the analyte is supplied; and a capture reagent site to which a capture reagent capable of specifically binding to and capturing a complex of the analyte and the labeled reagent has been immobilized, wherein at least one functional site having a particular or multiple functions is provided between the specimen-supply site and the capture reagent site.
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