A two-wire serial handshaking communication interface and a method thereof is provided for a transmission of a data bit representative between a first transmitting component and a second transmitting component, wherein the first transmitting component includes a first input/output unit and a second input/output unit and the second transmitting component has a third input/output unit and a fourth input/output unit, wherein the first input/output unit and the second input/output unit transmit a first signal through a first transmitting line electrically connected therebetween and the third input/output unit and the fourth input/output unit transmit a second signal transmitted through a second transmitting line electrically connected therebetween without strict constraint on communication timing.