A method for optimizing an operation of at least one wind turbine includes defining a plurality of test parameters that include a plurality of test points for at least one wind turbine operational parameter, wherein each test point includes a plurality of test values for the wind turbine operational parameter, and defining at least one test sequence of the plurality of test points. The method also includes initiating a test that executes the plurality of test points within the at least one randomized test sequence and measures at least one operating condition of the at least one wind turbine at each test point.