Invention Grant
- Patent Title: Selective amplification of methylated nucleic acids
- Patent Title (中): 甲基化核酸的选择性扩增
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Application No.: US11658569Application Date: 2005-09-02
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Publication No.: US07989169B2Publication Date: 2011-08-02
- Inventor: Frank Bergmann , Christine Markert-Hahn
- Applicant: Frank Bergmann , Christine Markert-Hahn
- Applicant Address: US CA Pleasanton
- Assignee: Roche Molecular Systems, Inc.
- Current Assignee: Roche Molecular Systems, Inc.
- Current Assignee Address: US CA Pleasanton
- Agent Rhea Cyd Nersesian
- Priority: EP04020974 20040903
- International Application: PCT/EP2005/009475 WO 20050902
- International Announcement: WO2006/024541 WO 20060309
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C12P19/34
Abstract:
The invention is related to a method for amplifying a methylated target nucleic acid in a sample while avoiding amplification of a non-methylated target nucleic acid by inactivating it. This is accomplished by a restriction enzyme digest after bisulfite treatment of the target nucleic acid. The invention is further related to the use of a restriction enzyme to avoid amplification of a non-methylated target nucleic acid while amplifying a methylated target nucleic acid in a sample and kits for performing the methods according to the invention.
Public/Granted literature
- US20090208930A1 Dna decontamination method Public/Granted day:2009-08-20
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