An image sensing apparatus includes an output unit including a first output line which transmits a first signal of the first pixel, a second output line which transmits a second signal of the first pixel, a third output line which transmits a first signal of the second pixel, a fourth output line which transmits a second signal of the second pixel, a first difference circuit which operates the difference between the first signal and the second signal of the first pixel to generate a first image signal, and a second difference circuit which operates the difference between the first signal and the second signal of the second pixel to generate a second image signal, wherein the first output line is arranged between the third output line and the fourth output line, and the third output line is arranged between the first output line and the second output line.