A signal shielding box with a plurality of shielding covers is provided. The shielding covers are pivotally connected with a shielding case and cover one another so as to respectively conceal or expose the opening. A connector is positioned at the shielding case and is electrically connected with the wireless communication device within the shielding case. The connector is electrically connected with a measurement and analysis device out of the shielding case. The shielding covers are respectively opened and closed so as to expose and conceal the opening. Thus, a plurality of shielding effects are formed so as to shield and measure signals of the wireless communication device.