Invention Grant
- Patent Title: Procedure for the determination of fragmentation of DNA in animal sperm
- Patent Title (中): 确定动物精子中DNA分裂的程序
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Application No.: US10586298Application Date: 2005-01-26
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Publication No.: US07993827B2Publication Date: 2011-08-09
- Inventor: Jaime Gosalvez Berenguer , Jose Luis Fernandez Garcia , Vicente Goyanes Villaescusa
- Applicant: Jaime Gosalvez Berenguer , Jose Luis Fernandez Garcia , Vicente Goyanes Villaescusa
- Applicant Address: ES Madrid
- Assignee: Universidad Autonoma de Madrid
- Current Assignee: Universidad Autonoma de Madrid
- Current Assignee Address: ES Madrid
- Agency: Ladas & Parry LLP
- Priority: ES200400163 20040126
- International Application: PCT/IB2005/000187 WO 20050126
- International Announcement: WO2006/079864 WO 20060803
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C07H21/02
Abstract:
The present invention describes a method for the determination of DNA fragmentation in animal sperm. It particularly refers to a procedure to evaluate the integrity of the chromatin/DNA of the sperm by means of a treatment of the sample with a denaturing solution followed, optionally by a stain; a subsequent treatment with a lysis solution that does not contain a protein denaturing detergent, followed, optionally, by a stain; and an evaluation of the integrity of the chromatin/DNA. The present invention also refers to a Kit to evaluate the quality of the sperm of animals which includes a DNA denaturing solution and a lysis solution that does not contain a protein denaturing detergent.
Public/Granted literature
- US20070281298A1 Procedure for the Determination of Fragmentation of Dna in Animal Sperm Public/Granted day:2007-12-06
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