Invention Grant
- Patent Title: Directed enrichment of genomic DNA for high-throughput sequencing
- Patent Title (中): 导向富集基因组DNA进行高通量测序
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Application No.: US12504485Application Date: 2009-07-16
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Publication No.: US07993842B2Publication Date: 2011-08-09
- Inventor: Kevin McKernan , Alan Blanchard , Douglas R. Smith
- Applicant: Kevin McKernan , Alan Blanchard , Douglas R. Smith
- Applicant Address: US CA Carlsbad
- Assignee: Life Technologies Corporation
- Current Assignee: Life Technologies Corporation
- Current Assignee Address: US CA Carlsbad
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C12P19/34
Abstract:
The present invention provides microarrays of oligonucleotide primer pairs, and in particular, microarrays of primers that comprise at least one cleavable linkage. Also provided are methods to capture oligonucleotide primer pairs from one or more microarrays, and methods to use the captured oligonucleotide primer pairs, such as for amplification of a target polynucleotide sequence. In addition, methods of using a microarray to isolate, purify and/or amplify a target polynucleotide are provided.
Public/Granted literature
- US20090280540A1 DIRECTED ENRICHMENT OF GENOMIC DNA FOR HIGH-THROUGHPUT SEQUENCING Public/Granted day:2009-11-12
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