A mouse with adjustable button activating pressure includes a body, two circuit boards, two switch sets and at least one adjusting means. The body is provided with two adjacent buttons. The two switch sets are provided in the body and electrically connected to the corresponding circuit board to correspond to the buttons of the body respectively. Each switch set includes a plurality of switches of different activating pressures. The adjusting means is adjustably assembled with the body and coupled to the switch set, thereby allowing the switch set to move with respect to the button of the body. Via the above arrangement of the present invention, the button activating pressure can be adjusted.