The invention relates to a punching tool for taking a biological sample from a filter using punching, the punching tool comprising a punch and a die provided with a punching channel for conveying the sample from the upper surface of the die towards the lower end of the die that includes a guidance means of the sample comprising a guidance channel arranged as an extension of the punching channel for receiving the sample from the punching channel, the diameter of the guidance channel is 1.02 to 2× the diameter of the punch and is greater than the diameter of the punching channel, and at the connecting point with the punching channel the guidance channel forms a shoulder and broadens towards the free end of the guidance means. In order to reduce the number of mistakes when taking samples, while the structure of the punching tool still remains simple at the end of the guidance means that faces towards the punching channel of the die, the diameter of the guidance channel is constant at a portion of the length thereof and the walls of the shoulder are placed at an angle from 170 to 300 degrees in relation to one another.