Invention Grant
US08012691B2 Multiplex compositions and methods for quantification of human nuclear DNA and human male DNA and detection of PCR inhibitors
有权
用于定量人核DNA和人类雄性DNA的多重组合物和方法以及PCR抑制剂的检测
- Patent Title: Multiplex compositions and methods for quantification of human nuclear DNA and human male DNA and detection of PCR inhibitors
- Patent Title (中): 用于定量人核DNA和人类雄性DNA的多重组合物和方法以及PCR抑制剂的检测
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Application No.: US12034634Application Date: 2008-02-20
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Publication No.: US08012691B2Publication Date: 2011-09-06
- Inventor: Jaiprakash G. Shewale , Manohar Furtado , Pius Brzoska , Maura Barbisin , Rixun Fang , Michael Malicdem , Cristin O'Shea
- Applicant: Jaiprakash G. Shewale , Manohar Furtado , Pius Brzoska , Maura Barbisin , Rixun Fang , Michael Malicdem , Cristin O'Shea
- Applicant Address: US CA Carlsbad
- Assignee: Applied Biosystems, LLC
- Current Assignee: Applied Biosystems, LLC
- Current Assignee Address: US CA Carlsbad
- Agent Shirley A. Recipon
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C12P19/34
Abstract:
The invention relates to a method for simultaneous quantification of human nuclear DNA and human male DNA in a biological sample while also detecting the presence of PCR inhibitors in a single reaction. The multiplex quantification method also provides a ratio of human nuclear and male DNA present in a biological sample. Such sample characterization is useful for achieving efficient and accurate results in downstream molecular techniques such as genotyping.
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