Invention Grant
- Patent Title: Methods for the production of hemagglutinating virus of Japan and adenovirus using cultured cells
- Patent Title (中): 使用培养细胞生产日本血凝素病毒和腺病毒的方法
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Application No.: US12083126Application Date: 2006-10-04
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Publication No.: US08012749B2Publication Date: 2011-09-06
- Inventor: Takahiro Yano , Tetsuji Nagasawa , Akiko Temma , Naho Suzuki , Kazue Miyaji
- Applicant: Takahiro Yano , Tetsuji Nagasawa , Akiko Temma , Naho Suzuki , Kazue Miyaji
- Applicant Address: JP Ibaraki-shi, Osaka
- Assignee: GenomIdea, Inc.
- Current Assignee: GenomIdea, Inc.
- Current Assignee Address: JP Ibaraki-shi, Osaka
- Agency: Law Office of: Michael A. Sanzo, LLC
- Priority: JP2005-292045 20051005
- International Application: PCT/JP2006/319849 WO 20061004
- International Announcement: WO2007/040242 WO 20070412
- Main IPC: C12N5/00
- IPC: C12N5/00 ; C12N7/00

Abstract:
A novel cell suited for mass production of Hemagglutinating Virus of Japan (HVJ), a method for obtaining the cell and use of the cell are disclosed. The human cell is originated from a transformed human kidney cell line, the doubling time thereof in logarithmic growth phase in suspension culture in a serum-free medium is not more than 40 hours, the cell has a freeze-recovery property, the maximum density of viable cells in suspension culture is not less than 106 cells/mL, and HVJ can grow in the cell. The method for obtaining the human cell comprises the steps of suspension-culturing a human transformed kidney cell line in a serum-free medium, and cloning the grown cells; and selecting, from the cloned cells, a cell whose doubling time in logarithmic growth phase in suspension culture in a serum-free medium is not more than 40 hours, which has a freeze-recovery property, whose maximum density of viable cells in suspension culture is not less than 106 cells/mL, in which HVJ can grow.
Public/Granted literature
- US20080318299A1 Isolated Human Cell, Method for Obtaining the Same and their Use Public/Granted day:2008-12-25
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