A novel apparatus comprising three main systems: air sampling, detection and computerized electric system; and method of using the same in the sampling, detection and identification of bioaerosols, wherein the identification of the said bioaerosol is base on a multiphoton laser diagnostic technique along with the velocity and aerodynamic size of the particular bioaerosol. After exposing the said bioaerosols with near infrared wavelength laser, the obtained fluorescence spectra has been shown to be unique and particular for each bioaerosol, thus allowing the characterization of the said particles.