A fender supporting structure of a two-wheeled motor vehicle capable of securing the rigidity of a mounting portion of a front fender and the rigidity of the entire front fender is provided, without enlarging the outer shape of the front fender more than necessary. In a fender supporting structure of a two-wheeled motor vehicle including a fender formed in an arc shape along the shape of a wheel, and a fender supporting member on which the fender is mounted from under the fender supporting member so as to cover an area above the wheel, at least two mounting surfaces at different levels in height are formed on the fender, at least two mounting surfaces at different levels in height are formed on the fender supporting member, and the fender is joined to the fender supporting member with these mounting surfaces.