The present invention provides an electrochemical sensing test piece without hemocyte interference, including a main body, electrode unit, reaction tank and chemical reaction zone. The detection zone of the electrode unit corresponds to the inserting end of the main body, and the reaction zone of the electrode unit corresponds to the sensing end of the main body. The reaction tank is arranged onto the sensing end correspondingly to the reaction zone of the electrode unit. The reaction tank is provided with a porous filter layer, whose aperture must be less than 6 μm for or separation of hemocyte in the blood sample. A chemical reaction zone is arranged between the porous filter layer and the reaction zone of the electrode unit. The hemocyte of the blood sample can be blocked and filtered by the porous filter layer, ensuring that the serum of blood sample can enter into the chemical reaction zone.