Invention Grant
US08026052B1 Delivery of nucleic acids into eukaryotic genomes using in vitro assembled Mu transposition complexes
有权
使用体外组装的Mu转座复合物将核酸递送到真核基因组中
- Patent Title: Delivery of nucleic acids into eukaryotic genomes using in vitro assembled Mu transposition complexes
- Patent Title (中): 使用体外组装的Mu转座复合物将核酸递送到真核基因组中
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Application No.: US10553353Application Date: 2004-04-14
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Publication No.: US08026052B1Publication Date: 2011-09-27
- Inventor: Harri Savilahti , Mikko Frilander , Xiaojuan Meng , Anja Paatero , Maria Pajunen , Hilkka Turakainen
- Applicant: Harri Savilahti , Mikko Frilander , Xiaojuan Meng , Anja Paatero , Maria Pajunen , Hilkka Turakainen
- Applicant Address: FI Espoo
- Assignee: Finnzymes Oy
- Current Assignee: Finnzymes Oy
- Current Assignee Address: FI Espoo
- Agency: Birch, Stewart, Kolasch & Birch, LLP
- Priority: FI20030561 20030414
- International Application: PCT/FI2004/000228 WO 20040414
- International Announcement: WO2004/090146 WO 20041021
- Main IPC: C12Q1/68
- IPC: C12Q1/68
Abstract:
The present invention relates to genetic engineering and especially to the use of DNA transposition complex of bacteriophage Mu. In particular, the invention provides a gene transfer system for eukaryotic cells, wherein in vitro assembled Mu transposition complexes are introduced into a target cell and subsequently transposition into a cellular nucleic acid occurs. The invention further provides a kit for producing insertional mutations into the genomes of eukaryotic cells. The kit can be used, e.g., to generate insertional mutant libraries.
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