Invention Grant
US08026078B2 Method of quantifying glycosylated protein using redox reaction and quantification kit
有权
使用氧化还原反应和定量试剂盒定量糖基化蛋白的方法
- Patent Title: Method of quantifying glycosylated protein using redox reaction and quantification kit
- Patent Title (中): 使用氧化还原反应和定量试剂盒定量糖基化蛋白的方法
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Application No.: US10501291Application Date: 2003-01-29
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Publication No.: US08026078B2Publication Date: 2011-09-27
- Inventor: Satoshi Yonehara , Kaori Ishimaru , Tsuguki Komori
- Applicant: Satoshi Yonehara , Kaori Ishimaru , Tsuguki Komori
- Applicant Address: JP Kyoto
- Assignee: ARKRAY Inc.
- Current Assignee: ARKRAY Inc.
- Current Assignee Address: JP Kyoto
- Agency: Morgan, Lewis & Bockius LLP
- Priority: JP2002-023897 20020131; JP2002-023898 20020131
- International Application: PCT/JP03/00820 WO 20030129
- International Announcement: WO03/064683 WO 20030807
- Main IPC: C12Q1/26
- IPC: C12Q1/26
Abstract:
The present invention provides a method of measuring a glycated protein in a sample using a redox reaction, by which the glycated protein can be measured accurately with high sensitivity. In order to remove a glycated amino acid present in the sample other than the glycated protein, the glycated amino acid is degraded in advance by causing a fructosyl amino acid oxidase to act thereon, and thereafter, a fructosyl amino acid oxidase is caused to act on the glycated protein in the presence of a tetrazolium compound and sodium azide to cause a redox reaction. The amount of the glycated protein is determined by measuring the redox reaction. As the glycated protein, glycated hemoglobin is preferable.
Public/Granted literature
- US20050042709A1 Method of quantifying glycosylated protein using redox reaction and quantification kit Public/Granted day:2005-02-24
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