A defrost indicator is configured such that the process of freezing and defrosting the defrost indicator places the defrost indicator into a state that is not completely reversed by refreezing, so that one can detect whether the defrost indicator defrosted and refroze or never defrosted by observing its current state. In an embodiment, the defrost indicator is initially placed in a first state before being frozen (e.g., upside down with fluid in an upper chamber). Freezing the defrost indicator places it in a second state (e.g., with the fluid frozen in the upper chamber). Then when the defrost indicator is defrosted, the defrost indicator is placed into a third state that is different than the first state (e.g., right-side-up with liquid fluid in the lower chamber). If the defrost indicator is again frozen, the defrost indicator is placed into a fourth state (e.g., the fluid frozen in the lower chamber).