Disclosed is a multi-layer film comprising: a surface layer having a thickness of 10 to 50 ∫Êm made of an ethylene-∫-olefin copolymer having a density of 0.935 to 0.950 g/cm3, a flexible layer having a thickness of 100 to 200 ∫Êm made of an ethylene-∫-olefin copolymer having a density of 0.860 to 0.930 g/cm3, a barrier layer having a thickness of 10 to 80 ∫Êm made of a mixed resin containing 60 to 95% by weight of a cyclic olefin polymer and 5 to 40% by weight of an ethylene-∫-olefin copolymer having a density of 0.900 to 0.965 g/cm3, and a seal layer having a thickness of 5 to 80 ∫Êm made of an ethylene-∫-olefin copolymer having a density of 0.910 to 0.950 g/cm3. This multi-layer film is suited for use as a material for production of a medicine container 10 because it suppresses adsorption of a medicine by the barrier layer and is also superior in strength and flexibility.