Invention Grant
- Patent Title: Lipid acyltransferases
- Patent Title (中): 脂质酰基转移酶
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Application No.: US12040721Application Date: 2008-02-29
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Publication No.: US08030044B2Publication Date: 2011-10-04
- Inventor: Jørn Borch Søe , Jørn Dalgaard Mikkelson , Arno de Kreij
- Applicant: Jørn Borch Søe , Jørn Dalgaard Mikkelson , Arno de Kreij
- Applicant Address: DK Copenhagen
- Assignee: Danisco A/S
- Current Assignee: Danisco A/S
- Current Assignee Address: DK Copenhagen
- Agency: Vedder Price P.C.
- Agent Thomas J. Kowalski; Heidi Lunasin
- Priority: GB0330016.7 20031224; GB0415999.2 20040716
- Main IPC: C12N9/10
- IPC: C12N9/10 ; C07H21/04

Abstract:
The present invention relates to a method of producing a variant lipid acyltransferase enzyme by selecting a parent enzyme which is a lipid acyltransferase enzyme having the amino acid sequence motif GDSX, modifying one or more amino acids to produce a variant lipid acyltransferase, testing the variant lipid acyltransferase for activity on a galactolipid substrate, a phospholipid substrate and/or a triglyceride substrate, selecting a variant enzyme with an enhanced activity towards galactolipids compared with the parent enzyme, and/or preparing a quantity of the variant enzyme. In some embodiments, the variant lipid acyltransferase enzyme may include the amino acid sequence motif GDSX, wherein X is one or more of the following amino acid residues L, A, V, I, F, Y, X, Q, T N, M or S, and wherein the variant enzyme has one or more amino acid modifications compared with a parent sequence.
Public/Granted literature
- US20090181124A1 PROTEINS Public/Granted day:2009-07-16
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