A hose for use in bio-pharmaceutical applications includes an innermost tubular layer made from a fluoropolymer material. The tubular layer has an inner, relatively smooth and pure surface that defines an opening for transfer of various media. A layer of silicone is disposed next to an outer surface of the innermost tubular layer through use of, for example, an extrusion method. The outer surface of the tubular layer may first be modified by various treatments or chemicals to facilitate the adhesion of the silicone layer thereto. The silicone material may be solid or foamed. A reinforcement layer may be disposed adjacent to an outer surface of the silicone layer. The reinforcement layer may be formed of a wire braid having gaps. An outer jacket of silicone may be attached to the reinforcement layer and to the inner silicone layer through the gaps in the reinforcement layer.