Invention Grant
US08055451B2 Method for estimating a melting temperature of a nucleic acid in buffers containing magnesium ions
有权
用于估计含有镁离子的缓冲液中核酸的解链温度的方法
- Patent Title: Method for estimating a melting temperature of a nucleic acid in buffers containing magnesium ions
- Patent Title (中): 用于估计含有镁离子的缓冲液中核酸的解链温度的方法
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Application No.: US11970509Application Date: 2008-01-07
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Publication No.: US08055451B2Publication Date: 2011-11-08
- Inventor: Richard Owczarzy , Bernardo Moreira , Yong You , Mark Aaron Behlke , Joseph Alan Walder
- Applicant: Richard Owczarzy , Bernardo Moreira , Yong You , Mark Aaron Behlke , Joseph Alan Walder
- Applicant Address: US IA Coralville
- Assignee: Integrated DNA Technologies, Inc.
- Current Assignee: Integrated DNA Technologies, Inc.
- Current Assignee Address: US IA Coralville
- Agency: McDonnell Boehnen Hulbert & Berghoff LLP
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; G01N33/50 ; G01N33/48 ; G06G17/00
Abstract:
The invention relates to methods and systems for predicting or estimating the melting temperature of duplex nucleic acids, in the presence of divalent cations, particularly duplexes of oligonucleotides which may be used as, for example, but not limited to primers or probes in PCR and/or hybridization assays. The methods and algorithms use novel formulas, having terms and coefficients that are functions of the particular nucleotide sequence, to estimate the effect of divalent cation salt conditions on the melting temperature.
Public/Granted literature
- US20090198453A1 METHOD FOR ESTIMATING A MELTING TEMPERATURE OF A NUCLEIC ACID IN BUFFERS CONTAINING MAGNESIUM IONS Public/Granted day:2009-08-06
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