A circuit for calibrating the DC offset in a wireless communication device utilizes a voltage-generating circuit to generate a first voltage value and its negative value, and utilizes a detecting circuit to detect an output of the wireless communication device and generate a first target-branch reference value corresponding to the power of the output when the first voltage value is inputted into a target branch (e.g., the in-phase branch or the quadrature branch) of the wireless communication device, and detect an output of the wireless communication device and generate a second target-branch reference value corresponding to the power of the output when the negative value of the first voltage value is input into the target branch. Then, an estimating circuit estimates the DC offset on the target branch according to the first and second target-branch reference values and the first voltage value.