Invention Grant
- Patent Title: Gene related to growth promoting function of acetic acid bacterium, acetic acid bacterium bred using the gene and method for producing vinegar using the acetic acid bacterium
- Patent Title (中): 与乙酸菌生长促进功能相关的基因,使用该基因繁殖的乙酸菌和使用乙酸菌生产醋的方法
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Application No.: US12281147Application Date: 2007-03-01
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Publication No.: US08067538B2Publication Date: 2011-11-29
- Inventor: Tomoo Kondo
- Applicant: Tomoo Kondo
- Applicant Address: JP
- Assignee: Mizkan Group Corporation
- Current Assignee: Mizkan Group Corporation
- Current Assignee Address: JP
- Agency: King & Spalding LLP
- Agent Kenneth H. Sonnenfeld; Margaret B. Brivanlou
- International Application: PCT/JP2007/053901 WO 20070301
- International Announcement: WO2007/100036 WO 20070907
- Main IPC: A61K38/00
- IPC: A61K38/00 ; A61K39/00 ; A61K39/02 ; C07K14/00 ; C07K17/00 ; C07K16/00 ; C07K2/00 ; C07K4/00 ; C07K5/00 ; C07K7/00 ; C07H21/04 ; C07H21/02 ; C12N1/12 ; C12N1/20

Abstract:
The present invention is to obtain a novel gene that encodes a protein having a function of promoting acetic acid bacterial growth in the presence of a high concentration of acetic acid; to enhance acetic acid bacteria's function of promoting growth and thereby to develop acetic acid bacteria with an improved fermentation ability in the presence of a high concentration of acetic acid; and to provide a method for efficiently producing vinegar that contains a high concentration of acetic acid in a short time using the acetic acid bacterium. A novel gene having a function of improving the function of promoting growth at a practical level was cloned from acetic acid bacteria for practical use, belonging to the genus Gluconacetobacter, by a method comprising exposing acetic acid bacterial lysate to the presence of a high concentration of acetic acid; obtaining a gene of a protein that was not insolubilized but remained soluble; and thereby obtaining a gene having a function of promoting acetic acid bacterial growth in the presence of acetic acid. When cultured in the presence of acetic acid and ethanol, a transformant of acetic acid bacteria transformed with the gene was confirmed to have a significant effect of promoting growth.
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