Invention Grant
- Patent Title: Methods for plant regeneration, transformation and production of insect resistant transgenic Okra
- Patent Title (中): 植物再生,转化和产生抗虫转基因秋葵的方法
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Application No.: US11574007Application Date: 2005-08-16
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Publication No.: US08067673B2Publication Date: 2011-11-29
- Inventor: Usha Barwale Zehr , Madhavan Narendran Nair , Satish Govindrao Deole
- Applicant: Usha Barwale Zehr , Madhavan Narendran Nair , Satish Govindrao Deole
- Applicant Address: IN
- Assignee: Maharashtra Hybrid Seeeds Company Ltd.
- Current Assignee: Maharashtra Hybrid Seeeds Company Ltd.
- Current Assignee Address: IN
- Agency: Lowe Hauptman Ham & Berner, LLP
- Priority: IN1522/DEL/2004 20040820
- International Application: PCT/IN2005/000277 WO 20050816
- International Announcement: WO2006/018857 WO 20060223
- Main IPC: C12N15/84
- IPC: C12N15/84 ; A01H5/00 ; A01H5/10 ; A01H1/02

Abstract:
The present description concerns methods for regeneration of whole plant from the explants obtained from the Abelmoschus species preferably A. esculentus. In addition the present description also concerns methods for transforming okra plant, plant cells and tissues either with the use of recombinant Agrobacterium strain or by bombarding the explants with tungsten or gold particles coated with DNA sequences of interest. An efficient method to isolate embryos from imbibed seeds of okra is also described which enables the use of young meristematic cells of plumule tip for efficient regeneration and transformation of okra plants. Further, transformed okra plants, plant cells and tissues for improved agronomic/non agronomic traits and insect resistance are produced either by using marker based or marker free systems.
Public/Granted literature
- US20090025099A1 METHODS FOR PLANT REGENERATION, TRANSFORMATION AND PRODUCTION OF INSECT RESISTANT TRANSGENIC OKRA Public/Granted day:2009-01-22
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