Disclosed is a pilot signal transmitting/receiving device and method in a wireless communication system. When generating a pilot signal block that is shorter than the length of a data signal, a frequency for each predetermined pilot signal block is transitioned so as to guarantee frequency domain channel estimation performance. A cyclic prefix according to the length of the transitioned pilot signal is added to the pilot signal, a data signal is time division multiplexed together with the pilot signal, and a resultant signal is transmitted through an antenna. In advance to this, a position of a subcarrier for transmitting the pilot signal and the data signal is determined, a data signal is mapped on the determined subcarrier, and in this instance, diversity on the channel for a symbol is used to map the data signal on the transmission method using one of the localized FDMA (L-FDMA) channel and the distributed FDMA (D-FDMA) channel. Therefore, the frequency resolution in the frequency domain is maintained to thus provide high-quality channel estimation performance.