A process cartridge detachably attached to an image forming apparatus includes a first subunit, a second subunit, a first positioning member, and a second positioning member. The first subunit accommodates a photoconductor therein. The second subunit accommodates a developer applicator therein. The photoconductor and the developer applicator are arranged substantially parallel to each other to define a development gap therebetween. The first positioning member is fastened to both the first and second subunits to position ends of the photoconductor and the developer applicator on a first side of the respective subunits. The second positioning member is fastened to only one of the first and second subunits to position ends of the photoconductor and the developer applicator on a second side of the respective subunits opposite to the first side. An image forming apparatus employing such a process cartridge is also disclosed.