A golf tee with a connecting wire includes a tee and a connecting wire. The tee has a nail rod and a ball holding portion connected with a top of the nail rod. The ball holding portion has a groove formed in a top thereof and a connecting slot formed in one side thereof. The connecting slot includes an opening portion, a passage portion and a fixing portion. One end of the connecting wire passes through the opening portion and the passage portion of the connecting slot into the fixing portion and forms a blocked portion which is located in the groove, so that the end of the connecting wire is connected with the tee. The present invention further provides a method of manufacturing a golf tee with a connecting wire. Accordingly, the connection and assembling between the connecting wire and the tee can be attained easily, firmly, and rapidly.