Invention Grant
US08092791B2 Method of producing herpes simplex virus amplicons, resulting amplicons, and their use
失效
产生单纯疱疹病毒扩增子，产生的扩增子及其用途的方法
- Patent Title: Method of producing herpes simplex virus amplicons, resulting amplicons, and their use
- Patent Title (中): 产生单纯疱疹病毒扩增子，产生的扩增子及其用途的方法
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Application No.: US10296551Application Date: 2001-05-23
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Publication No.: US08092791B2Publication Date: 2012-01-10
- Inventor: Howard J. Federoff , William J. Bowers
- Applicant: Howard J. Federoff , William J. Bowers
- Applicant Address: US NY Rochester
- Assignee: University of Rochester
- Current Assignee: University of Rochester
- Current Assignee Address: US NY Rochester
- Agency: McKeon, Meunier, Carlin & Curfman LLC
- International Application: PCT/US01/16682 WO 20010523
- International Announcement: WO01/89304 WO 20011129
- Main IPC: A01N63/00
- IPC: A01N63/00 ; C12N15/64 ; C12N5/00 ; C12N15/00
Abstract:
The present invention relates to a method for producing herpes simplex virus (HSV) amplicon particles which includes co-transfecting a host cell with the following: (i) an amplicon vector comprising an HSV origin of replication, an HSV cleavage/packaging signal, and a heterologous transgene expressible in a patient, (ii) one or more vectors individually or collectively encoding all essential HSV genes but excluding all cleavage/packaging signals, and (iii) a vhs expression vector encoding a virion host shutoff protein; and then isolating HSV amplicon particles produced by the host cell, the HSV amplicon particles including the transgene. Also disclosed are a system and a kit for preparing HSV amplicon particles, HSV amplicon particles prepared according to the process of the present invention, and their use.
Public/Granted literature
- US20040105844A1 Method of producing herpes simplex virus amplicons, resulting amplicons, and their use Public/Granted day:2004-06-03
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