A backlight module and a wire positioning/holding device for the frame structure thereof are disclosed. The backlight module mainly includes a frame and a light source module. The light source module is disposed on the lateral side or on the bottom of the frame. The wire holding device mainly includes a main body and a winding pillar. A first guide slot is disposed on the main body, and the first guide slot has an inlet end and an outlet end. The winding pillar extends from the main body and is situated at one side of the first guide slot. The winding pillar and the main body together form a wire holding portion. The wire holding portion is disposed corresponding to the outlet end of the first guide slot to receive the wires of the light source module coming out of the outlet end. Moreover, the wire holding portion and the outlet end of the first guide slot are situated on opposite sides of the winding pillar.