Invention Grant
- Patent Title: Microparticle-based transfection and activation of dendritic cells
- Patent Title (中): 基于微粒的树突状细胞的转染和活化
-
Application No.: US12755266Application Date: 2010-04-06
-
Publication No.: US08097243B2Publication Date: 2012-01-17
- Inventor: John J. Donnelly , Kimberly S. Denis-Mize , Gary S. Ott
- Applicant: John J. Donnelly , Kimberly S. Denis-Mize , Gary S. Ott
- Applicant Address: US CA Emeryville
- Assignee: Novartis Vaccines and Diagnostics, Inc.
- Current Assignee: Novartis Vaccines and Diagnostics, Inc.
- Current Assignee Address: US CA Emeryville
- Agent Helen Lee; David Bonham
- Main IPC: A61K48/00
- IPC: A61K48/00 ; C12N15/63 ; C12N5/0783 ; C12N5/0784
Abstract:
The present invention provides an effective method for the transfection of dendritic cells by non-viral methods. The present invention provides this benefit by incubating dendritic cells and a specified transfection agent. The transfection agent comprises a polynucleotide and microparticles, with the microparticles being comprised of biodegradable polymer and cationic detergent. The dendritic cells and transfection agent are incubated for a time sufficient to transfect the dendritic cells with the polynucleotide.
Public/Granted literature
- US20100196339A1 Microparticle-Based Transfection and Activation of Dendritic Cells Public/Granted day:2010-08-05
Information query
