Invention Grant
US08097404B2 Composition and method for cell permeabilization comprising N-octyl-β-D-glucopyranoside, sodium polyphosphates, rubidium chloride and/or lithium chloride for detecting living cells on a membrane
有权
用于细胞透化的组合物和方法，包括N-辛基-β-D-吡喃葡萄糖苷，多磷酸钠，氯化铷和/或氯化锂，用于检测膜上的活细胞
- Patent Title: Composition and method for cell permeabilization comprising N-octyl-β-D-glucopyranoside, sodium polyphosphates, rubidium chloride and/or lithium chloride for detecting living cells on a membrane
- Patent Title (中): 用于细胞透化的组合物和方法，包括N-辛基-β-D-吡喃葡萄糖苷，多磷酸钠，氯化铷和/或氯化锂，用于检测膜上的活细胞
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Application No.: US12383277Application Date: 2009-03-20
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Publication No.: US08097404B2Publication Date: 2012-01-17
- Inventor: Sebastien Ribault , Frederic Olivieri , Herve Meder , David Lehmann , Sandra Laborde
- Applicant: Sebastien Ribault , Frederic Olivieri , Herve Meder , David Lehmann , Sandra Laborde
- Applicant Address: US MA Billerica
- Assignee: Millipore Corporation
- Current Assignee: Millipore Corporation
- Current Assignee Address: US MA Billerica
- Agency: Nields, Lemack & Frame, LLC
- Priority: FR0852139 20080401
- Main IPC: C12Q1/00
- IPC: C12Q1/00
Abstract:
The present invention relates to a composition for the permeabilization of the walls of microorganisms comprising the combination of octyl-β-D-glucopyranoside (NOG), sodium polyphosphates (HMP), and a salt chosen from lithium chloride or rubidium chloride, and a method for the enumeration and identification of cells on a membrane using said composition.
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