Disclosed is a thin film chemical analysis apparatus and an analysis method using the same, which can solve the sealing problem caused by environmental factors (e.g. impact, temperature) for long circulation and storage periods, as well as can detect an analyte quickly and easily. The thin film chemical analysis apparatus includes at least one chamber adapted to store a fluid necessary for biological or biochemical analysis or to conduct a biological or biochemical reaction; channels for fluid-connection of the chambers; holes arranged between or inside the channels and connected to the channels; a rotatable body having the chambers, the channels, and the holes integrated into the body; and a burst valve having a sealing means for closing the hole and sealing a fluid in the chamber, the sealing means being torn away from the hole by centrifugal force resulting from rotation of the body and opening the hole.