A method of determining parameter values used in impedance analysis of a subject. The method includes using a processing system to determine a number of impedance measurements at a corresponding number of frequencies. The impedance measurements are used to determine estimates of parameter values, with the estimates being used to determine theoretical impedance values based on the parameter value estimates. The theoretical impedance values are compared to the measured impedance values to allow a modification direction to be determined for at least one of the parameter value estimates. This is then used to modify at least one parameter value estimate, with the parameter values being determined at least in part from one or more modified parameter value estimates.